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PanaceaGel

About

Scaffold plays a key role in cell proliferation in a three-­dimensìonal cell culture. Collagen, a well-known scaffold, is widely used in this field. However, its animal­-derived nature and variability may contribute to variations in experimental data.

To solve this problem, We developed PanaceaGel as a superior 3D cell culture alternative to Collagen.PanaceaGel does not include any animal-derived ingredients. instead, PanaceaGel is composed of 100% synthetic peptides, which self-assemble to form a nanofiber-scale three-dimensional network in aqueous solvent.

Primary Structure

The main component of PanaceaGel is a thirteen amino acid synthetic peptide, which is made of four naturally occurring amino acids in a specific sequence (US 2012/0058066 A1). The primary structure is:

Primary Structure
R:Arginine(Basic), L:Leucine(neutral・hydrophobic)
D:Aspartic acid(acidic), A:Alanine(neutral・hydrophobic)

Mechanism of Gel Formation

The thirteen amino acid peptides undergo spontaneous self-assembly in several steps to form nanotibers in aqueous solvents due to hydrogen bonding, electrostatic interaction and hydrophobic interaction. These nanofibers assemble into a higher-order structure, a nanofiber-scale three-dimensional network, which forms a transparent gel with a moisture content of over 99%.

Mechanism of Gel Formation
ßsheet structure
Thirteen amino acid peptides form ß sheets due to hydrogen bonding,
electrostatic interactions and hydrophobic interactions.
  Nanofiber formation
Each β sheet has two sides: a hydrophilic surface and a hydrophobic surface. β sheets stack to each other to form nanofibers in aqueous solvent due to hydrophobic interactions.
  Nanofiber network structure
Nanofibers interact with each other to form a network containing more than 99% water.

Product Summary

PanaceaGel is a 100% synthetic peptide hydrogel with a neutral pH range. It could be used as a scaffold in three-dimensional cell culture and as a carrier for drug delivery. lt does not contain any animal-derived or biologically active components, thus increasing experimental reproducibility compared to Collagen-based 3D cell cultures.

The main component of PanaceaGel is a thirteen amino acid peptide, composed of four types of naturally occurring amino acids, arginine, leucine, aspartic acid and alanine.
The peptides self-assemble into a three-dimensional network to form a hydrogel in aqueous solvent.

PanaceaGel is in a neutral pH range. Therefore, no pre-treatment is necessary before being used with a cell suspension.

Intended Use

Three-dimensional cell culture scaffold, drug delivery carrier, tissue engineering, and more.

Three-Dimensional Cell Culture

細胞3次元培養
Three-dimensional cell culture in PanaceaGel
A. Mouse skeletal muscle cell (C2C12) proliferation (day 8). Blue: DAPI staining,
Green: Calcein staining. Scale bar = 50μm.
B. Human chondrosarcoma cell (OUMS-27) immunostaining (day 12).
Blue: DAPI staining, Green: agrecan, Red: Type II collagen. Scale bar = 10μm.

PanaceaGel has been successfully used in the three-dimensional culture of mouse skeletal muscle cell (C2C12, see image A), human chondrosarcoma cell (OUMS-27, see image B), rat skeletal muscle cell (L6), isolated rat neonatal cardiac myocyte, and human fetal lung fibroblast (TIG-l ).


<Reference>
1_ Nagai Y, Yokoi H, Kaihara K, Naruse K. The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel. Biomaterials 2012;33(4):1044-1051

Characteristics

  • ・100% synthetic peptide gel scaffold
  • ・No animal-derived components or pathogens
  • ・Transparent and neutral pH range
  • ・Ready to use
  • ・Isotonic options available (SPG-178-204 and SPG-178-208)
  • ・Store at room temperature

<Comparison Sheet>

PanaceaGel Collagen Biodegradable polymer
Origin Synthetic Animal-derived Synthetic
Stiffness Middle Middle Hard
pH Neutral Neutral・Acidic Neutral
Biocompatibility Good Excellent Good
Transparency High High Low



<Product Specifications>

Catalog Number SPG-178-004 SPG-178-204 SPG-178-008 SPG-178-208
Peptide
concentration
0.4 w/w% 0.8 w/w%
pH Neutral
Isotonic Option No Yes No Yes
Autoclaved Yes
Volume 1mL/2mL


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